An In-situ Mutational Analysis of BCL-2 Gene in Pets Cancer

Abstract

To cure various cancers in veterinary medicine, the BCL-2 gene and its polymorphisms are very well known due to its anti-apoptotic and anti-cancerous activity. In the current study, different polymorphisms of the BCL-2 gene were studied to find a cure for lymphoma, TVT, and other cancers in dogs and cats. Various parameters are used in the present study, which aims to investigate coding regions of Bcl-2gene for analyzing cancer mutations. Different in-vivo and in-silico tools, including DNA extraction, PCR, spectrophotometry, NEB cutter online tool, and BLAST, were used to analyze the blood samples of 15 dogs and cats to find any association between BCL-2 and cancers. However, no significant result was reported, and couldn’t find any link between polymorphisms of the BCL-2 gene with lymphoma, mammary gland tumor, oral tumor, peritoneal adenocarcinoma, and transmissible venereal tumor (TVT) in exons 1 and 2. In conclusion, further study with much larger blood and tissue samples is required to determine the polymorphisms and accurately identify the associated factors. There is a need to explore the other gene mutations causing cancers in the pet population, which will ultimately help develop genetic counselling strategies, gene therapies, and prenatal diagnostic procedures for the pet population.